APA
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Rebaque, D., Carrasco-López, C., Krishnan, P., López, G., López-Cobos, S., de Salas, F., … Sánchez-Vallet, A. (2024). Zymoseptoria tritici stealth infection is facilitated by stage-specific down-regulation of a β-glucanase. BioRxiv.
Chicago/Turabian
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Rebaque, Diego, Cristian Carrasco-López, Parvathy Krishnan, G. López, Sergio López-Cobos, Felipe de Salas, L. Meile, et al. “Zymoseptoria Tritici Stealth Infection Is Facilitated by Stage-Specific down-Regulation of a β-Glucanase.” bioRxiv (2024).
MLA
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Rebaque, Diego, et al. “Zymoseptoria Tritici Stealth Infection Is Facilitated by Stage-Specific down-Regulation of a β-Glucanase.” BioRxiv, 2024.
BibTeX Click to copy
@article{diego2024a,
title = {Zymoseptoria tritici stealth infection is facilitated by stage-specific down-regulation of a β-glucanase},
year = {2024},
journal = {bioRxiv},
author = {Rebaque, Diego and Carrasco-López, Cristian and Krishnan, Parvathy and López, G. and López-Cobos, Sergio and de Salas, Felipe and Meile, L. and Lorrain, Cécile and Largo-Gosens, Asier and McDonald, Bruce A. and Vilaplana, Francisco and Martínez, María Jesús and Mélida, Hugo and Molina, Antonio and Sánchez-Vallet, A.}
}
Plant cell walls constitute a major defence barrier against pathogens, although it is unclear how specific cell wall components impact pathogen colonisation. Pathogens secrete cell wall degrading enzymes (CWDEs) to facilitate plant colonisation, but damaged, infected cells are often a source of cell wall-derived oligosaccharides that trigger host immunity. The mechanisms by which pathogens minimize the release of cell wall-derived oligosaccharides while colonizing the host remain to be elucidated. We combined biochemical, molecular genetics and transcriptomic analyses to functionally characterize a glycoside hydrolase (ZtGH45) from the wheat pathogen Zymoseptoria tritici. ZtGH45 gene is expressed during the necrotrophic phase of the fungus, coinciding with an accumulation of wheat β-1,3/1,4-mixed-linked glucan (MLG)-derived oligosaccharides. We show that overexpression of ZtGH45 enhances β-1,3/1,4-glucan hydrolysis and the derived oligosaccharides trigger an immune response in wheat, which hinders Z. tritici virulence. The results demonstrate that tight regulation of ZtGH45 is critical for the infection process to prevent early accumulation of MLG oligosaccharides that would prematurely induce host immunity counterbalancing fungal virulence. We suggest that the balance between plant cell wall degradation by fungal CWDE and the release of immunogenic wall-derived oligosaccharides governs the outcome of host invasion by pathogens.