FISIOVEGEN ULE - Title : Changes In Cell Wall Polymers And

Journal article

S. Fornalé, J. Rencoret, L. García-Calvo, A. Encina, J. Rigau, Ana, Gutiérrez, J. C. Río, D. Caparros-Ruiz
2016

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APA Click to copy
Fornalé, S., Rencoret, J., García-Calvo, L., Encina, A., Rigau, J., Ana, … Caparros-Ruiz, D. (2016). Title : Changes In Cell Wall Polymers And Degradability In Maize Mutants Lacking 3 ′-And 5 ′-O-Methyltransferases Involved In Lignin Biosynthesis Running head : Effects of COMT and CCoAOMT 1 knockout in maize.

Chicago/Turabian Click to copy
Fornalé, S., J. Rencoret, L. García-Calvo, A. Encina, J. Rigau, Ana, Gutiérrez, J. C. Río, and D. Caparros-Ruiz. “Title : Changes In Cell Wall Polymers And Degradability In Maize Mutants Lacking 3 ′-And 5 ′-O-Methyltransferases Involved In Lignin Biosynthesis Running Head : Effects of COMT and CCoAOMT 1 Knockout in Maize” (2016).

MLA Click to copy
Fornalé, S., et al. Title : Changes In Cell Wall Polymers And Degradability In Maize Mutants Lacking 3 ′-And 5 ′-O-Methyltransferases Involved In Lignin Biosynthesis Running Head : Effects of COMT and CCoAOMT 1 Knockout in Maize. 2016.

BibTeX Click to copy

@article{s2016a,
  title = {Title : Changes In Cell Wall Polymers And Degradability In Maize Mutants Lacking 3 ′-And 5 ′-O-Methyltransferases Involved In Lignin Biosynthesis Running head : Effects of COMT and CCoAOMT 1 knockout in maize},
  year = {2016},
  author = {Fornalé, S. and Rencoret, J. and García-Calvo, L. and Encina, A. and Rigau, J. and Ana and Gutiérrez and Río, J. C. and Caparros-Ruiz, D.}
}

Abstract

Caffeoyl Coenzyme A 3-O-Methyltransferase (CCoAOMT) and Caffeic acid-O-Methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits. The function of these enzymes was characterised in single and double mutant maize plants. In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound. In contrast, the ccoaomt1 mutants displays wild type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound. Based on our data, we suggest that CCoAOMT1 is involved in lignin biosynthesis at least in midribs. The phenotype of ccoaomt1 mutant plants displays no alterations, and their lignin content and composition remain unchanged. On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to the ones already described for the comt mutant. Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, being the cell walls of the ccoaomt1 the most degradable. This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio. In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.